Mouse MAPt (Microtubule Associated Protein Tau) ELISA Kit - ELK2999

Mouse MAPt (Microtubule Associated Protein Tau) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2999-48, ELK2999-96, ELK2999-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Tau Protein; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway; Neuroscience; Developmental science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MAPt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MAPt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MAPt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MAPt in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P10637

Research Use Only