Product Description

Mouse MASP1 (Mannose Associated Serine Protease 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10478-48, ELK10478-96, ELK10478-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CRARF, PRSS5, Mannan-Binding Lectin Serine Peptidase 1, Ra-reactive factor serine protease p100, Complement-activating component of Ra-reactive factor, Serine protease 5

Reactivity: Mouse

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Metabolic Pathway, Apoptosis, Infection Immunity, Hematology

Assay Length: 3.5h

Sensitivity: 6.7 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

UniProt: P98064

Research Use Only