Product Description

Mouse MIP1g (Macrophage Inflammatory Protein 1 Gamma) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7798-48, ELK7798-96, ELK7798-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: CCL9, CCL10, SCYB9, CCF18, MRP2, C-C motif chemokine 9, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9

Sensitivity: 3.2 pg/mL

Assay Type: Sandwich

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Cytokine, Infection Immunity, Human Gene Deletion

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1g in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only