Mouse Monoclonal Antibody to Human CDNF - 302-100

Mouse Monoclonal Antibody to Human CDNF

Size: 100 µg

Catalogue Number: 302-100

Citations, Manuals and MSDS Available upon request.

Immunogen: Human CDNF

Immunogen Description: Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant

Background: CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

UniProt ID: Q49AH0

Alternative Names: ARMETL1

Clonality: Mouse monoclonal

Clone: 6G5

Class: IgG1

Reactivity: Human, no reactivity with mouse CDNF Binds to the C-terminal part of the human CDNF (aa 126-187)

Application: ELISA, WB, IF, IHC

Protocol: ELISA - 0.06 to 0.2 µg/ml; WB - 0.25 to 1 µg/ml; IF - 0.3 to 10 µg/ml; IHC (on formalin-fixed, paraffin-embedded tissues, antigen retrieval) - 5 to 10 µg/ml;
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification: Protein G purified

Buffer: PBS pH 7.4, with 0.1% sodium azide

Shipping: This product is shipped in non-frozen liquid form in ambient conditions

Storage: Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Research Use Only