Mouse Monoclonal Antibody to Human MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor) - 311-100

Mouse Monoclonal Antibody to Human MANF
(Mesencephalic Astrocyte-Derived Neurotrophic Factor)

Size: 100 µg

Catalogue Number: 311-100

Citations, Manuals and MSDS Available upon request.

Immunogen: Human MANF

Immunogen Description: Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant

Background: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

UniProt ID: P55145

Alternative Names: ARMET, ARP

Clonality: Mouse monoclonal

Clone: 1G12

Class: IgG1

Reactivity: Human MANF

Application: ELISA, WB, IF

Protocol: ELISA 0.1 to 0.2 µg/ml; WB 0.5 to 2 µg/ml; IF 1 to 10 µg/ml
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification: Protein G purification

Buffer: PBS pH 7.4, with 0.1% sodium azide

Shipping: This product is shipped in non-frozen liquid form in ambient conditions

Storage: Store at -20°C to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Research Use Only