Mouse NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10410-48, ELK10410-96, ELK10410-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: NT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 0.31 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Areas: Endocrinology; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only