Product Description

Mouse NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK5445-48, ELK5445-96, ELK5445-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: NT-Pro-BNP, , N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb

Reactivity: Mouse

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Endocrinology, Cardiovascular Biology

Assay Length: 3.5h

Sensitivity: 28 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Research Use Only