Mouse NTS (Neurotensin) ELISA Kit - ELK0079

Mouse NTS (Neurotensin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0079-48, ELK0079-96, ELK0079-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NEUT; neuromedin N; NN; NT; NT/N; NTS1; NMN-125

Assay Type: Competitive Inhibition

Sensitivity: 12.2 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NTS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NTS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NTS in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9D3P9

Research Use Only