Mouse PAI2 (Plasminogen Activator Inhibitor 2) ELISA Kit - ELK10452

Mouse PAI2 (Plasminogen Activator Inhibitor 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10452-48, ELK10452-96, ELK10452-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor

Assay Type: Sandwich

Sensitivity: 0.16 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: plasma

Assay Length: 3.5h

Research Areas: Hematology; Reproductive science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P12388

Research Use Only