Mouse PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK11219-48, ELK11219-96, ELK11219-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1
Assay Type: Competitive Inhibition
Sensitivity: 6.3 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.6-1000 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Areas: Signal transduction
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only