Product Description

Mouse PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK2231-48, ELK2231-96, ELK2231-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PGG/HS, PGHS2, PHS2, HCox2, COX2, Cyclooxygenase 2, Prostaglandin G/H Synthase And Cyclooxygenase, Prostaglandin H2 synthase 2, PTGS2, Prostaglandin Endoperoxide Synthase 2

Reactivity: Mouse

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Tumor Immunity, Infection Immunity, Reproductive Science, Pulmonology, Gastroenterology, Hormone Metabolism

Assay Length: 3.5h

Sensitivity: 35 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

UniProt: Q05769

Research Use Only