Product Description

Mouse Rock1 (Rho Associated Coiled Coil Containing Protein Kinase 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0322-48, ELK0322-96, ELK0322-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Rock1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Rock1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Rock1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Rock1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: coiled-coil-containing protein kinase 2, KIAA0619, p164 ROCK 2, p164 ROCK-2, Rho associated coiled coil containing protein kinase 2, Rho associated protein kinase 2, Rho associated, coiled coil containing protein kinase II, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, ROCK 2, Rock II, Rock2, Rock2m, ROK alpha, ROKalpha

Reactivity: Mouse

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Enzyme and Kinase, Tumor Immunity, Infection Immunity

Assay Length: 3.5h

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: P70335

Research Use Only