Mouse S1PR3 (Sphingosine 1 Phosphate Receptor 3) ELISA Kit - ELK7242

Mouse S1PR3 (Sphingosine 1 Phosphate Receptor 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7242-48, ELK7242-96, ELK7242-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: EDG3; LPB3; S1P3; S1PR3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; Sphingosine 1-phosphate receptor Edg-3

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S1PR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S1PR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S1PR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S1PR3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9Z0U9

Research Use Only