Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit - ELK9883

Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9883-48, ELK9883-96, ELK9883-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Smad 2/3

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates

Assay Length: 3.5h

Research Areas: Signal transduction; Apoptosis; Tumor immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Smad2/3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only