Mouse T3 (Triiodothyronine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10141-48, ELK10141-96, ELK10141-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
Assay Type: Competitive Inhibition
Sensitivity: 0.12 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Areas: Endocrinology; Autoimmunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only