Mouse TACR1 (Tachykinin Receptor 1) ELISA Kit - ELK0600

Mouse TACR1 (Tachykinin Receptor 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0600-48, ELK0600-96, ELK0600-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NK1R; SPR; NKIR; TAC1R; Neurokinin 1 Receptor; Substance P Receptor

Assay Type: Sandwich

Sensitivity: 0.073 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Neuroscience

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TACR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TACR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TACR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TACR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P30548

Research Use Only