Mouse TNFa (Tumor Necrosis Factor Alpha) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1387MS-48, ELK1387MS-96, ELK1387MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: Tp44
Assay Type: Sandwich
Sensitivity: 0.32 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: serum, plasma, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD28. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD28. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD28, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD28 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P10747
Research Use Only