Mouse TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit - ELK9616

Mouse TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9616-48, ELK9616-96, ELK9616-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ACP5; TRAP5b; SPENCDI

Assay Type: Sandwich

Sensitivity: 0.46 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Immunology; Cardiovascular

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only