Mouse UCN3 (Urocortin 3) ELISA Kit - ELK7478

Mouse UCN3 (Urocortin 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7478-48, ELK7478-96, ELK7478-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SCP; SPC; UCNIII; Stresscopin

Assay Type: Competitive Inhibition

Sensitivity: 4.78 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse UCN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse UCN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse UCN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q924A4

Research Use Only