Mouse VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit - ELK10552

Mouse VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10552-48, ELK10552-96, ELK10552-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5

Assay Type: Sandwich

Sensitivity: 1.21 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P55284

Research Use Only