Mouse VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit - ELK1078

Mouse VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1078-48, ELK1078-96, ELK1078-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Flt1; Fms-Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-like tyrosine kinase 1; Tyrosine-protein kinase receptor FLT

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; Tumor immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGFR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VEGFR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P35969

Research Use Only