Mouse VGLUT1 (Vesicular Glutamate Transporter 1) ELISA Kit - ELK7060

Mouse VGLUT1 (Vesicular Glutamate Transporter 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7060-48, ELK7060-96, ELK7060-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SLC17A7; BNPI; Solute Carrier Family 17 Member 7,Sodium-Dependent Inorganic Phosphate Cotransporter; Brain-specific Na (+)-dependent inorganic phosphate cotransporter

Assay Type: Sandwich

Sensitivity: 0.127 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VGLUT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VGLUT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VGLUT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VGLUT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q3TXX4

Research Use Only