Product Description

MYH Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2874-50, ES2874-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: MUTYH, MYH, A/G-specific adenine DNA glycosylase, MutY homolog, hMYH

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human MUTYH. AA range:151-200

Isotype: IgG

Target: MYH

Specificity: MYH Polyclonal Antibody detects endogenous levels of MYH protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Kidney,

Observed Band (KD): 60kD

Fields: Base excision repair

Function: Cofactor: Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand
Disease: Defects in MUTYH are a cause of autosomal recessive colorectal adenomatous polyposis [MIM: 608456]
Disease: Defects in MUTYH are a cause of gastric cancer [MIM: 137215]
Function: Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities
Similarity: Belongs to the nth/mutY family
Similarity: Contains 1 nudix hydrolase domain

Subcellular Location: Nucleus Mitochondrion

Storage and Stability: -20°C/1 year

Gene Name: MUTYH

Protein Name: A/G-specific adenine DNA glycosylase

Mouse Gene ID: 70603

Mouse SwissProt Number: Q99P21

Rat Gene ID: 170841

Rat SwissProt Number: Q8R5G2

Research Use Only