Product Description

NAD-Malic Enzyme (NAD-ME) Activity
Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K556-M-48, E-BC-K556-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Malic enzyme (ME) is widely present in both eukaryotes and prokaryotes and participates in various metabolic pathways. It mainly catalyzes the oxidation decarboxylation of malic acid to produce pyruvate and CO2, accompanied by the reduction of NAD (P) + to NAD (P) H. In mammalian cells, malic acid mainly includes ME1 in the cytoplasm that depends on NADP+, ME2 in the mitochondria that depends on NAD+, and ME3 in the mitochondria that depends on NADP+.
The detection principle of this kit: Malic enzyme catalyzes the substrate reaction to generate NADH. There is a characteristic absorption peak at 340 nm. By measuring the increase rate of absorbance at 340 nm, the activity of NAD-ME can be characterized.

Abbreviation: NAD-ME

Research Areas: Tricarboxylic Acid（TCA）Cycle,Mitochondria Function,Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 16.36-157.10 U/L

Tested Sample Types: Animal tissue;Plant tissue;Cell;Serum;Plasma

Assay Length: 30 min

Sensitivity: 16.36 U/L

Inter cv: 7.8%-9.9%

Intra cv: 2.9%-4.8%

Research Use Only