Product Description

NADP-Malic Enzyme (NADP-ME) Activity Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K555-M-48, E-BC-K555-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Malic enzyme (ME) is widely present in eukaryotes and prokaryotes and participates in multiple metabolic pathways. Its main metabolic substrate is malic acid, which mainly catalyzes the oxidation and decarboxylation of malic acid to pyruvate and CO2, accompanied by the reduction of NAD(P)+ to NAD(P)H. Malic acid is an important intermediate product in the tricarboxylic acid cycle. Under the catalysis of ME, malic acid is converted into pyruvate and NAD(P)H to regulate cellular energy, REDOX balance and the synthesis of biological macromolecules. In mammalian cells, malic enzymes mainly consist of NADP+ dependent ME1 in the cytoplasm, NAD+ dependent ME2 in the mitochondria, and NADP+ dependent ME3 in the mitochondria. Inhibiting ME1 can induce cellular senescence or apoptosis to suppress the growth of tumor cells.
The detection principle of this kit: The enzyme catalyzes the substrate reaction to generate NADPH, which has a characteristic absorption peak at 340 nm. The activity of NADP-ME can be determined by measuring the rate of increase in absorbance at 340 nm.

Abbreviation: NADP-ME

Research Areas: Tricarboxylic Acid（TCA）Cycle,Mitochondria Function,Energy Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 2.68-338.96 U/L

Tested Sample Types: Animal tissue;Plant tissue;Cell

Assay Length: 30 min

Sensitivity: 2.68 U/L

Inter cv: 1.5%-2.7%

Intra cv: 1.3%-1.4%

Research Use Only