Product Description

NADP+/NADPH Colorimetric Assay Kit (WST-8)

Sizes: 96T

Catalogue Numbers: E-BC-K803-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Detect total content of NADP+and NADPH:
Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+and NADPH can be quantified by measure the OD value at 450 nm.
Detect NADPH:
After treating sample, heat at 60℃ water bath for 30 min. the NADP+of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm.
Detect NADP+and NADP+/NADPH:
The content of NADP+and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+and NADPH obtained of the first two steps as well as the separate content of NADPH.

Target Synonym: NADP;NADPH

Research Areas: Oxidative Stress;Metabolic Diseases;Glycolysis and Lipid Metabolism;Cuproptosis;Disulfidptosis

Result Type: Quantitative

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.02-5.0 μmol/L

Tested Sample Types: Animal tissue;Cell

Assay Length: 1 h 10 min

Sensitivity: 0.02 μmol/L

Inter cv: 5.5%

Intra cv: 2.1%

Research Use Only