Product Description

NADPH Fluorometric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-F170-48, E-BC-F170-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Glucose-6-phosphate (G6P) is oxidized by glucose-6-phosphate dehydrogenase (G6PDH) to gluconolactone-6-phosphate (6-PG). At the same time, NADP+is reduced to NADPH in this reaction. The generated NADPH reacts with a chromogenic agent under enzymatic catalysis to produce a fluorescent substance. The fluorescence intensity can be measured to calculate the NADPH content in the sample.

Abbreviation: NADPH

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 8.55-1000 nmol/L

Tested Sample Types: Animal tissue;Cell

Assay Length: 2 h

Sensitivity: 8.55 nmol/L

Inter cv: 3.3%-8.4%

Intra cv: 1.7%-5.0%

Research Use Only