Native Microorganism Phosphoenolpyruvate Carboxylase - DIA-212

Native Microorganism Phosphoenolpyruvate Carboxylase

Size: 3 KU

Catalogue Number: DIA-212

Citations, Manuals and MSDS Available upon request.

Product Description: Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases found in plants and some bacteria that catalyzes the addition of bicarbonate (HCO3−) to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate and inorganic phosphate: PEP + HCO3-→ oxaloacetate + Pi. This reaction is used for carbon fixation in CAM (crassulacean acid metabolism) and C4 organisms, as well as to regulate flux through the citric acid cycle (also known as Krebs or TCA cycle) in bacteria and plants. The enzyme structure and its two step catalytic, irreversible mechanism have been well studied. PEP carboxylase is highly regulated, both by phosphorylation and allostery.

Storage: -20°C

Applications: This enzyme is useful for enzymatic determination of carbon dioxide when coupled with malate dehydrogenase in clinical analysis.

Activity: GradeⅢ 5.0U/mg-solid or more

CAS#: 9067-77-0

Research Use Only