Product Description

NCK1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES9576-50, ES9576-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Target: NCK1

Specificity: NCK1 Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Epithelium, Platelet, Uterus,

Observed Band (KD): 41kD

Fields: ErbB signaling pathway, Axon guidance, T cell receptor signaling pathway, Pathogenic Escherichia coli infection

Function: Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1
PTM: Phosphorylated on Ser and Tyr residues
Similarity: Contains 1 SH2 domain
Similarity: Contains 3 SH3 domains
Subunit: Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1

Subcellular Location: Cytoplasm Endoplasmic reticulum Nucleus Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus Import into the nucleus requires the interaction with SOCS7 Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments

Storage and Stability: -20°C/1 year

Gene Name: NCK1 NCK

Protein Name: Cytoplasmic protein NCK1 (NCK adaptor protein 1) (Nck-1) (SH2/SH3 adaptor protein NCK-alpha)

Mouse SwissProt Number: Q99M51

Research Use Only