Product Description

Neutrophil Elastase (NE) Activity Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K899-M-48, E-BC-K899-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Neutrophil Elastase (NE) is one of the main serine proteases secreted by neutrophils. It plays an important role in the physiological and pathological processes of the body, on the one hand NE participate in the formation of the body's defense system and assist in the elimination of pathogenic microorganisms. On the other hand, the excessive activation of NE, in the case of imbalance with endogenous inhibitors, is directly involved in tissue damage, and is closely related to the occurrence of a variety of inflammation-related diseases, such as atherosclerosis, chronic obstructive pulmonary disease, pulmonary cystic fibrosis and other diseases, which can be used as a prevention and treatment target for these diseases.
The detection principle of this kit is as follows: NE hydrolyzates the substrate to generate chromogenicity substances, which have maximum absorption at the wavelength of 380 nm, and NE activity in the sample is calculated by measuring the OD value at 380 nm and the standard curve.

Abbreviation: NE

Research Areas: Amino Acids and Proteins

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.54-28.57 U/L

Tested Sample Types: Animal tissue

Assay Length: 1 h 20 min

Sensitivity: 0.54 U/L

Inter cv: 6.3-7.5%

Intra cv: 4.1-5.0%

Research Use Only