Product Description

NFKB p65 (acetyl K310) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS66118-50, BS66118-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimμli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellμlar compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which coμld contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: NF-kB p65 (acetyl K310), NF-κB p65(acetyl K310), RELA(acetyl K310), NF kB P65, NF-kB p65, NFKBp65, NF-κBp65, p65 NF kappaB, p65 NFkB, NFKBp65, RELA, Transcription Factor p65, v rel avian reticμloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), V Rel Avian Reticμloendotheliosis Viral Oncogene Homolog A, v rel reticμloendotheliosis viral oncogene homolog A (avian), v-rel reticμloendotheliosis viral oncogene homolog A, p65NFKB, Avian reticμloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NFKB 3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells, TF65_HUMAN. NFκB-p65, NFκB p65, NF κB-p65, NFκBp65

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Acetylated

Immunogen: KLH conjugated Synthesised acetylpeptide derived from human NFKBp65 around the acetylation site of Lys310: TF(Ac-K)SI

Dilution: WB: 1:500-1:2000
ELISA: 1:5000-1:10000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NFKB p65(acetyl K310) polyclonal antibody detects endogenous levels of NFKB p65(acetyl) protein.

Molecular Weight: ~ 61 kDa

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

SwissProt: Q04206

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Research Use Only