Product Description

NM23-H2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6396-50, ES6396-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: NME2, NM23B, Nucleoside diphosphate kinase B, NDK B, NDP kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, nm23-H2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human NM23. AA range:91-140

Isotype: IgG

Target: NM23-H2

Specificity: NM23-H2 Polyclonal Antibody detects endogenous levels of NM23-H2 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: [Isoform 1]: Ubiquitously expressed,, [Isoform 3]: Ubiquitously expressed,

Observed Band (KD): 23kD

Fields: Purine metabolism, Pyrimidine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Nucleotide metabolism, Biosynthesis of cofactors

Function: Catalytic Activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Cofactor: Magnesium
Disease: This protein is found in reduced amount in tumor cells of high metastatic potential
Disease: This protein is found in reduced amount in tumor cells of high metastatic potential. Somatic mutations of NME1 are found in neuroblastoma. Increased NME1 in neuroblastoma is correlated with features of the disease that are associated with aggressive tumors. May therefore have distinct if not opposite roles in different tumors
Enzyme Regulation: Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity
Function: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the

Subcellular Location: Cytoplasm Cell projection, lamellipodium Cell projection, ruffle Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates, [Isoform 1]: Cytoplasm Cytoplasm, perinuclear region Nucleus, [Isoform 3]: Cytoplasm Cytoplasm, perinuclear region Nucleus

Storage and Stability: -20°C/1 year

Gene Name: NME2

Protein Name: Nucleoside diphosphate kinase B

Mouse Gene ID: 18103

Mouse SwissProt Number: Q01768

Rat Gene ID: 83782

Rat SwissProt Number: P19804

Research Use Only