Product Description

NRF-1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6416-50, ES6416-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014],

Reactivity: Human, Mouse, Rat, Bovine

Host: Rabbit

Applications: WB, ELISA

Alternate Names: NRF1, Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human NRF1. AA range:221-270

Isotype: IgG

Target: NRF-1

Specificity: NRF-1 Polyclonal Antibody detects endogenous levels of NRF-1 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitously expressed with strongest expression in skeletal muscle,

Observed Band (KD): 54kD

Fields: Apelin signaling pathway, Huntington disease

Function: Function: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication
PTM: Phosphorylation enhances DNA binding
Similarity: Belongs to the NRF1/Ewg family
Subunit: Homodimer. Binds DNA as a dimer. Interacts with PPRC1
Tissue Specificity: Ubiquitously expressed with strongest expression in skeletal muscle

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: NRF1

Protein Name: Nuclear respiratory factor 1

Mouse Gene ID: 18181

Mouse SwissProt Number: Q9WU00

Rat SwissProt Number: Q62792

Research Use Only