Product Description

p130 Cas Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3094-50, ES3094-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: BCAR1, CAS, CASS1, CRKAS, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, p130cas

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human p130 Cas. AA range:376-425

Isotype: IgG

Target: p130 Cas

Specificity: p130 Cas Polyclonal Antibody detects endogenous levels of p130 Cas protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed with an abundant expression in the testis, Low level of expression seen in the liver, thymus, and peripheral blood leukocytes, The protein has been detected in a B-cell line,

Observed Band (KD): 130kD

Fields: Rap1 signaling pathway, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Growth hormone synthesis, secretion and action, Bacterial invasion of epithelial cells, Shigellosis, Yersinia infection, Human cytomegalovirus infection

Function: Domain: A serine-rich region promotes activation of the serum response element (SRE)
Domain: Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL
Domain: The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1
Function: Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells
PTM: Focal adhesion kinase 1 phosphoryl

Subcellular Location: Cell junction, focal adhesion Cytoplasm Cell projection, axon Unphosphorylated form localizes in the cytoplasm (By similarity) Localizes to focal adhesion sites following integrin engagement (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: BCAR1

Protein Name: Breast cancer anti-estrogen resistance protein 1

Mouse Gene ID: 12927

Mouse SwissProt Number: Q61140

Rat Gene ID: 25414

Rat SwissProt Number: Q63767

Research Use Only