Product Description

p16 INK4a Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS66210-50, BS66210-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The progression of cells through the cell cycle is regμlated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Mμltiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resμlting in the production of the p19 ARF protein in mice and the p14 ARF protein in humans. p14 ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14 ARF is negatively regμlated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF/ICC

Alternate Names: CDKN2, MTS1, Cyclin-dependent kinase inhibitor 2A isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A, CDK4I, Mμltiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: KLH-conjugated synthetic peptide of human p16 INK4a.

Dilution: WB: 1:500~1:2000
IHC: 1:50~1:200
IF/ICC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p16 INK4a Polyclonal Antibody detects endogenous levels of p16 INK4a protein.

Molecular Weight: ~ 17 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P42771

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only