Product Description

PAF65α (Q53) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2175-50, BS2175-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9Y6J9

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: In eukaryotic systems, the process of initiating transcription from protein-coding genes requires the presence of RNA polymerase II and a broad family of auxiliary transcription factors. TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element. Functioning as a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs), TFIID mediates promoter responses to various transcriptional activators and repressors. TAF6L, also known as PAF65A, is a 622 amino acid nuclear protein that functions as part of the PCAF (p300/CBP-associated factor) histone acetylase complex.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PAF65α (Q53) polyclonal antibody detects endogenous levels of PAF65α protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PAF65α (Q53) Antibody at 1:1000 dilution
Lane 1: HepG2 whole cell lysate (10ug)
Lane 2: HEK293T whole cell lysate (10ug)
Lane 3: A549 whole cell lysate (10ug)
Lane 4: EC9706 whole cell lysate (10ug)
Lane 5: The Lung tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of PAF65α (Q53) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.

Bioworld Molecular Weight: ~ 68 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L; PCAF-associated factor 65-alpha; PAF65-alpha; TAF6L; PAF65A

Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human PAF65α.

Conjugate: Unconjugated

Modification: Unmodified