Pan-Akt Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A18120-20, A18120-100
Citations, Manuals and MSDS Available upon request.
Background: Human AKT serine-threonine protein kinase family includes three members AKT1, AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K) . AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4, 5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 48kDa/55kDa/51kDa/54kDa
Observed Molecular Weight: 60kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 207/208/10000
SWISS: P31749/P31751/Q9Y243
Alternate Names: AKT1/AKT2/AKT3; Pan-Akt
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 IF/ICC, 1:50 - 1:100 IP, 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Protein phosphorylation.
NCBI Alias: AKT
Research Use Only