Pan-Ubiquitin Remnant Motif (K-ε-GG) Polyclonal Antibody-BS74124

Pan-Ubiquitin Remnant Motif (K-ε-GG) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74124-50, BS74124-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Ubiquitination plays a key role in protein degradation and signal transduction. Characterization of ubiquitination sites is important for understanding the role of this modification in cellular processes and disease. Ubiquitination sites are usually identified by detection of Lys-?-Gly-Gly (K-?-GG)-remnant peptides, which are generated by tryptic digestion of proteomes. The di-glycine remnant left at sites of ubiquitination after trypsin digestion through cleavage of the C-terminal –RGG sequence on ubiquitin (K-ε-GG). This Ubiquitin K-ε-GG remnant motif antibody will recognize the di-glycine remnant independent of flanking amino acid sequence.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Other Modified

Bioworld Molecular Weight: 15-100KDa

Immunogen: A synthetic peptide corresponding to a sequence containing ubiquitinated Motif(K-ε-GG)

Conjugate: Unconjugated

Modification: Other Modified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using pan-Ubiquitin Remnant Motif antibody at 1:1000 dilution. HeLa cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.