Product Description

PDGFRα Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91039-50, BS91039-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16234(Human) P26618(Mouse) P20786(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-β gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PDGFRα polyclonal antibody detects endogenous levels of PDGFRα protein.

Bioworld Molecular Weight: 123 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1,000 dilution., ICC staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PDGFR-alpha, PDGFR-2, PDGFRA, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a, CD140a antigen, MGC74795, PDGF alpha chain, PDGF-R-alpha, PDGFR 2, PDGFR alpha, PDGFR2, PDGFRA, PDGFRA/BCR fusion, PGFRA, Platelet derived growth factor receptor 2, Platelet derived growth factor receptor alpha, Platelet derived growth factor receptor alpha polypeptide, Platelet derived growth factor receptor, Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein, RHEPDGFRA,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified