PDK3 Polyclonal Antibody - BT-AP12905

PDK3 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP12905-20, BT-AP12905-50, BT-AP12905-100

Citations, Manuals and MSDS Available upon request.

Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research Areas: Metabolism; Signal transduction

Target Protein: PDK3

Applications: WB, ELISA

Reactivity: Human, Mouse

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

UniProt Accession: Human: Q15120; Mouse: Q922H2

Synonyms: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial;EC 2.7.11.2;Pyruvate dehydrogenase kinase isoform 3

GeneID: Human: 5165

Research Use Only