Product Description

PDZ-RhoGEF Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3173-50, ES3173-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: ARHGEF11, KIAA0380, Rho guanine nucleotide exchange factor 11, PDZ-RhoGEF

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ARHGEF11. AA range:198-247

Isotype: IgG

Target: PDZ-RhoGEF

Specificity: PDZ-RhoGEF Polyclonal Antibody detects endogenous levels of PDZ-RhoGEF protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitously expressed,

Molecular Weight (Da): 168kD

Fields: Vascular smooth muscle contraction, Parathyroid hormone synthesis, secretion and action, Pathogenic Escherichia coli infection, Human cytomegalovirus infection, Pathways in cancer

Function: Domain: The poly-Pro region is essential for plasma membrane localization upon stimulation
Function: May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13
Similarity: Contains 1 DH (DBL-homology) domain
Similarity: Contains 1 PDZ (DHR) domain
Similarity: Contains 1 PH domain
Similarity: Contains 1 RGSL (RGS-like) domain
Subcellular Location: Translocated to the membrane upon stimulation
Subunit: Interacts with GNA12 and GNA13 through the RGS domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts with SLC1A6
Tissue Specificity: Ubiquitously expressed

Subcellular Location: Cytoplasm Membrane Translocated to the membrane upon stimulation

Storage and Stability: -20°C/1 year

Gene Name: ARHGEF11

Protein Name: Rho guanine nucleotide exchange factor 11

Rat Gene ID: 78966

Rat SwissProt Number: Q9ES67

Research Use Only