PGF2a (Prostaglandin F2 Alpha) ELISA Kit - ELK7850

PGF2a (Prostaglandin F2 Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7850-48, ELK7850-96, ELK7850-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PGF2A; PGF2-A; PG-F2a

Assay Type: Competitive Inhibition

Sensitivity: 3.95 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Cardiovascular biology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only