Product Description

Phospho-AMPKβ1-S182 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS74219-50, BS74219-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a regμlatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellμlar energy status. In response to cellμlar metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regμlating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regμlator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellμlar localization of AMPK. This subunit may also serve as an adaptor molecμle mediating the association of the AMPK complex.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: PRKAB1, AMPK, HAMPKb

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphorylated peptide around S182 of human AMPKβ1(NP_006244.2).

Dilution: WB, 1:500 - 1:2000 IHC, 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Phosphorylated

Molecular Weight: 38KDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9Y478

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only