Product Description

Phospho-Band 3 (Tyr359) Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS43225-50, BS43225-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disμlphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubμlar acidosis. Other mutations that do not give rise to disease resμlt in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) resμlts from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One nμll mutation in this gene is known, resμlting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jμl 2008]

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: DI, FR, SW, WD, WR, AE1, CHC, SAO, WD1, BND3, EPB3, SPH4, CD233, EMPB3, RTA1A

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding
Tyr359 of human Band 3

Dilution: WB: 1:5000

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 102 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P02730

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only