Product Description

Phospho-ESRα-S118 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79351-50, BS79351-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P03372

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IF/ICC,1:100 - 1:200

Background: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 66kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of MCF7 cells, using Phospho-ESRα-S118 pAb at 1:1000 dilution.MCF7 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-ESRα-S118 antibody. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-ESRα-S118 antibody.

Alternative Name: ESR1; ER; ESR; ESRA; ESTRR; Era; NR3A1; ER alpha

Immunogen: A synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1)(NP_000116.2).

Conjugate: Unconjugated

Modification: Phosphorylated