Product Description

Phospho-GSK3β-Y216 + GSK3α-Y279 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS74004-50, BS74004-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49841

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-GSK3β-Y216 + GSK3α-Y279 antibody at 1:1000 dilution. 293T cells were treated by Insulin for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Bioworld Molecular Weight: 50kDa

Specificity: Phosphorylated Antibodies

Alternative Name: GSK3B; gsk-3β

Immunogen: A synthetic phosphorylated peptide around Y216 of human GSK3β(NP_001139628).

Conjugate: Unconjugated

Modification: Phosphorylated