Product Description

Phospho-PKA alpha/beta/gamma (catalytic subunit) (Thr197) Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS43274-50, BS43274-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regμlatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regμlatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regμlatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellμlar processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing resμlts in mμltiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, ICC/IF, IP

Alternate Names: PKACA, PPNAD4

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding
Tyr197 of human PKA alpha/beta/gamma (catalytic subunit)

Dilution: WB: 1:1000 IHC: 1:20-1:50 ICC/IF: 1:20-1:100 IP: 1:20

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 41 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P17612

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only