Product Description

Phosphocreatine (PCr) Fluorometric Assay Kit

Sizes: 96T

Catalogue Numbers: E-BC-F154-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Phosphocreatine (PCr) is a high-energy phosphate compound that mainly exists in muscle and nerve tissues and is an important substance in the rapid energy supply system of living organisms. It plays a buffering and energy storage role in cellular energy metabolism through its interaction with adenosine triphosphate (ATP), especially providing immediate energy to muscles during short-term high-intensity exercise.
The detection principle of this kit: The products generated by PCr through a series of enzymatic reactions cause the fluorescent probe to produce fluorescence. Fluorescence can be detected at an excitation wavelength of 535 nm and an emission wavelength of 587 nm. The concentration of PCr in the sample is calculated based on the magnitude of the fluorescence value.

Abbreviation: PCr

Research Areas: Energy Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Detection Type: Fluorometric method

Detection Range: 1.17-100.00 μmol/L

Tested Sample Types: Animal tissue;Cell

Assay Length: 1 h

Sensitivity: 1.17 μmol/L

Inter cv: 8.3-9.7%

Intra cv: 1.3-3.5%

Research Use Only