Product Description

Phospholipase C (PLC) Activity Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K781-M-48, E-BC-K781-M-96

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Phospholipase C (PLC) is a hydrolase that breaks down phosphatidylinositol diphosphate (PIP2) to form diacylglycerol (DAG) and inositol triphosphate (IP3). PLCS are widely found in bacteria, plants and animals. Its abnormal expression is associated with a variety of cancers, and studying the PLC mechanism can help develop new anticancer therapies. PLC also play a key role in nerve signaling and protection, helping to understand and treat neurodegenerative diseases. In addition, PLC plays an important role in insulin signaling and lipid metabolism, making it a hot topic in diabetes and obesity research.
PLC catalyzes the substrate to produce chromogenic substance has maximum absorption at 410 nm, and the PLC enzyme activity is calculated by measuring OD value at 410 nm.

Abbreviation: PLC

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.383-16.588 U/L

Tested Sample Types: Serum;Plasma;Animal and plant tissue;Cell

Assay Length: 1 h 10 min

Sensitivity: 0.383 U/L

Inter cv: 6.7-9.9%

Intra cv: 1.8-3.0%

Research Use Only