Product Description

pHyde Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES3196-50, ES3196-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: STEAP3 metalloreductase(STEAP3) Homo sapiens This gene encodes a multipass membrane protein that functions as an iron transporter. The encoded protein can reduce both iron (Fe3+) and copper (Cu2+) cations. This protein may mediate downstream responses to p53, including promoting apoptosis. Deficiency in this gene can cause anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: STEAP3, TSAP6, Metalloreductase STEAP3, Dudulin-2, Six-transmembrane epithelial antigen of prostate 3, Tumor suppressor-activated pathway protein 6, hTSAP6, pHyde, hpHyde

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human STEA3. AA range:421-470

Isotype: IgG

Target: pHyde

Specificity: pHyde Polyclonal Antibody detects endogenous levels of pHyde protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas, Down-regulated in hepatocellular carcinoma,

Observed Band (KD): 54kD

Fields: p53 signaling pathway, Ferroptosis

Function: Caution: Was initially thought to have tumor suppressor function in prostate cancer. However, it was shown that it is probably not the case (PubMed: 12866033)
Cofactor: FAD
Function: Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor (By similarity). May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP
Induction: By p53/TP53
Similarity: Belongs to the STEAP family
Similarity: Contains 1 ferric oxidoreductase domain
Subcellular Location: Localizes to vesicular-like structures at the plasma membrane and around the nucleus
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Subcellular Location: Endosome membrane, Multi-pass membrane protein Localizes to vesicular-like structures at the plasma membrane and around the nucleus

Storage and Stability: -20°C/1 year

Gene Name: STEAP3

Protein Name: Metalloreductase STEAP3

Mouse Gene ID: 68428

Mouse SwissProt Number: Q8CI59

Rat Gene ID: 170824

Rat SwissProt Number: Q5RKL5

Research Use Only